Thursday, November 18, 2010

Missing peaks

Hello,

I had a mutation in my protein and now I have some amino acids around mutation remained unassigned (as below): On the other hand, I have some peaks that I can not assign to the missing amino acids. As you can see below, I have put labels E, Q and R on some of them. Part of the missing sequence is L227, Q228. I have two suggestions for L227 that are E & Q; and one suggestion for Q228 that is R. Do you think this prediction is probable?


the strips are HNCACO, HNCA, CBCACONH, HNCO, HNCACA(ONLY IN E225 BUT NOT VISIBLE), HBHACONH, HCCH-TOCSY(ONLY IN K226)

Thanks

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